SARS-COV-2 was already spreading in France in late December 20196 May, 2020
The COVID-19 epidemic is believed to have started in late January 2020 in France. We report here a case of a patient hospitalized in December 2019 in our intensive care, of our hospital in the north of Paris, for hemoptysis with no etiological diagnosis and for which RT-PCR was performed retrospectively on the stored respiratory sample which confirmed the diagnosis of COVID-19 infection. Based on this result, it appears that the COVID-19 epidemic started much earlier.
Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform6 May, 2020
Reverse genetics has been an indispensable tool revolutionising insights into viral pathogenesis and vaccine development. Large RNA virus genomes, such as from Coronaviruses, are cumbersome to clone and manipulate in E. coli due to size and occasional instability1–3. Therefore, an alternative rapid and robust reverse genetics platform for RNA viruses would benefit the research community. Here we show the full functionality of a yeast-based synthetic genomics platform to genetica...
Site-specific glycan analysis of the SARS-CoV-2 spike5 May, 2020The emergence of the betacoronavirus, SARS-CoV-2, the causative agent of COVID-19, represents a significant threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, the scie...
SARS-CoV-2 productively infects human gut enterocytes5 May, 2020The virus severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission via the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), ...
Management Considerations for the Surgical Treatment of Colorectal Cancer During the Global Covid-19 Pandemic5 May, 2020Surgical practice guidelines for colorectal cancer must be modified for the unprecedented COVID-19 pandemic as well as potential future outbreaks. Data to support delays in surgical care are limited. This review published in Annals of Surgery analyzes management recommendations and the strength of published evidence using the GRADE system to provide a rational basis for clinical decision-making.
Colorectal cancer surgeries - prioritized as emergency, urgent with (a) imminent emergency or (...
A human monoclonal antibody blocking SARS-CoV-2 infection5 May, 2020
The emergence of the novel human coronavirus SARS-CoV-2 in Wuhan, China has caused a worldwide epidemic of respiratory disease (COVID-19). Vaccines and targeted therapeutics for treatment of this disease are currently lacking. Here we report a human monoclonal antibody that neutralizes SARS-CoV-2 (and SARS-CoV) in cell culture. This cross-neutralizing antibody targets a communal epitope on these viruses and may offer potential for prevention and treatment of COVID-19.
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Management Considerations for the Surgical Treatment of Colorectal Cancer During the Global Covid-19 Pandemic5 May, 2020
Surgical practice guidelines for colorectal cancer must be modified for the unprecedented COVID-19 pandemic as well as potential future outbreaks. Data to support delays in surgical care are limited. This review published in Annals of Surgery analyzes management recommendations and the strength of published evidence using the GRADE system to provide a rational basis for clinical decision-making.
Colorectal cancer surgeries - prioritized as emergency, urgent with (a) imminent emerge...
Contamination of Health Care Personnel During Removal of Personal Protective Equipment4 May, 2020Abstract
Importance: Contamination of the skin and clothing of health care personnel during removal of personal protective equipment (PPE) contributes to dissemination of pathogens and places personnel at risk for infection.
Objectives: To determine the frequency and sites of contamination on the skin and clothing of personnel during PPE removal and to evaluate the effect of an intervention on the frequency of contamination.
Design, setting, and participants: W...
SARS-CoV-2 is present in peritoneal fluid in COVID-19 patients4 May, 2020Abstract
The excretion patho-mechanisms of SARS-CoV-2 are actually unknown. No certain data exist about viral load in the different body compartments and fluids during the different disease phases.
In this study, specific real-time reverse transcriptase–polymerase chain reaction targeting three SARS-CoV-e genes were used to detect the presence of the virus.
Findings show that SARS-CoV-2 is present in peritoneal fluid at a higher concentration than in respiratory tract. ...
Imbalanced host response to SARS-CoV-2 drives development of COVID-194 May, 2020Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here, we offer an indepth analysis of the transcriptional response to SARS-CoV-2 as it compares to other respiratory viruses. Cell and animal models of SARS-CoV-2 infections, in addition to transcriptional and serum profiling of COVID-19 patients, consistentl...
A SARS-CoV-2 protein interaction map reveals targets for drug repurposing1 May, 2020
The novel coronavirus SARS-CoV-2, the causative agent of COVID-19 respiratory disease, has infected over 2.3 million people, killed over 160,000, and caused worldwide social and economic disruption. There are currently no antiviral drugs with proven clinical efficacy, nor are there vaccines for its prevention, and these efforts are hampered by limited knowledge of the molecular details of SARS-CoV-2 infection. To address this, these scientifics cloned, tagged and expressed 26 of the 29 SA...
Immediate and long-term impact of the COVID-19 pandemic on delivery of surgical services1 May, 2020Abstract
The ongoing pandemic is having a collateral health effect on delivery of surgical care to millions of patients. Very little is known about pandemic management and effects on other services, including delivery of surgery. In this article, a scoping review of all available literature pertaining to COVID‐19 and surgery, using electronic databases, society websites, webinars and preprint repositories was performed.
Several perioperative guidelines have been issued within a short ti...